The protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in 1640 RPMI
Clom 15 ATCC FAQ #11058. No PMID Leukemia, chronic myeloid. CVCL_8439. HL-60. Human. Leukemia, acute myeloid, M2. Feb 23, 2018 In addition, curcumin induced G1 phase arrest in HL-60 cells and G2/M the American Type Culture Collection (ATCC; Manassas, VA, USA).
Links to corresponding Cellosaurus descriptions and Research Resource Identifiers (RRID) have been added. The Cellosaurus identifier is a combination of the cell line name variant chosen by Cellosaurus, with the Cellosaurus accession ID, the latter written as RRID. > ATCC > hl60 cells. hl60 cells ATCC is a verified supplier ATCC manufactures this product . About; News; Press Release; Team; Advisors; Partners; Contact; Bioz Stars; Bioz vStars; 99. Name: HL 60. Description: Applications This cell line is a suitable transfection host Cell Type promyeloblast Host Homo sapiens human.
The HL-60 cell line is positive for myc oncogene expression. Cultures can be maintained by addition of fresh medium or replacement of medium (RPMI-1640
No. 15338-100). 7klvsurgxfwlvlqwhqghgiruoderudwru\uhvhdufksxusrvhvrqo\ ,wlvqrwlqwhqghgiruxvhlqkxpdqv :kloh$7&&xvhvuhdvrqdeohhiiruwvwrlqfoxghdffxudwhdqgxs wr Green columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with N , N -dimethylformamide (DMF). Gray columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with DMF and activated with phorbol 12-myristate 13-acetate (PMA). Values are means and SD of three independent experiments ( *** p Organism Type: Human: This is a non-adherent human leukocyte cell line.
THE NCI-60 PANEL. Cell lines with an ATCC number. DNA. Protein. ref. consensus. comment HL-60, CCL-240, R248L, 1018. p53 null. null, p.M1_* 394del
… I got a HL-60 cells from ATCC and cultured them in IMEM high glucose+20% FBS (non heat inactivated as recommend by atcc technical support)and cryopreserved the cells at a density if 4*105 cells/ml. Eos-HL-60 . 96100920 .
Blood 54: 713-733, 1979. … HL-60/MX1 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood leukocytes obtained by leukopheresis from patient with acute promyelocytic leukemia. Comments Subsequent exposure of the HL-60/MX1 cells to higher concentrations of mitoxantrone led to the emergence of cells capable of growth at 190 nM (see ATCC … HL-60/MX2 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood leukocytes obtained by leukopheresis from patient with acute promyelocytic leukemia. HL-60/MX1 (see ATCC CRL-2258) cells were selected and subcloned in 1987 for resistance to 39 nM mitoxantrone, an anthracenedione antitumor agent. Subsequent exposure of the … DNA taken from HL-60 (ATCC® CCL-240 ™) Cell Line.
HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e. HL-60 from ATCC Description HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e. HL-60 cells(CCL-240; ATCC, Manassas, VA) were maintained at 37°C in Iscove modifiedDulbecco medium supplemented with 10% (vol/vol) FBS and 1% (vol/vol) penicillin-streptomycin in a humidified atmosphere of 95% air and 5% CO2 Differentiation of HL-60 cells into PMN-like cells was induced by incubating them in 1.25% DMSO for 6 days.
ATCC offers exosomes derived from well … Article Snippet: The Ltx-sensitive human promyelocytic leukemia cell line HL-60 (ATCC CCL 240; American Type Culture Collection, Bethesda, Md.), was used for all experiments. Techniques: Incubation, Plasmid Preparation. Journal: Infection and Immunity. Article Title: Actinobacillus actinomycetemcomitans Leukotoxin Induces Apoptosis in HL-60 Cells.
Materials List 1. DMEM with 2mM L-glutamine (cellgro Cat# 10-013-CM) 2.alterar senha google účet
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ATCC recommends seeding HL-60 cells at a density of 1.0-2.0 x 10 Date Updated: 2/21/2014; Models for Normal Differentiation. The following ATCC cell lines can serve as models for normal differentiation: Date Updated: 6/5/2018; References Gallagher R, et al. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood 54: 713-733, 1979. …